English dropdown-ico

Manual Agilent Technologies G1978B

44 pages 0.99 mb
Download

Go to site of 44

Summary
  • Agilent Technologies G1978B - page 1

    Agilent T echnologies Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide ...

  • Agilent Technologies G1978B - page 2

    2 Multimode Source for 6100 Series Singl e Quad LC/MS Set-U p Guide Notices © Agilent T echnologies, Inc. 2008 N o p a rt o f t h i s m a n u a l m a y be r e p r o d u c e d i n any form or by any means (including elec- tronic storage and retrieval or translation into a foreign language) without prior agree- ment and written consen t from Agilent ...

  • Agilent Technologies G1978B - page 3

    Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3 In This Guide This guide explains how to install, maint ain and troubleshoot your nanoelectospray ion sour ce. 1 Installation Thi s c ha pte r tel ls y ou ho w to i ns tall the mul ti mod e i on so ur ce . 2 Ve r i f i c a t i o n Thi s c ha pte r tel ls y ou ho w to i ns tall the mu ...

  • Agilent Technologies G1978B - page 4

    4 Multimode Source for 6100 Series Singl e Quad LC/MS Set-U p Guide ...

  • Agilent Technologies G1978B - page 5

    Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 5 Content 1 Installation 7 Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cables 9 Changing Sources 13 T o remove the m ultimode source 13 T o convert from multimode to ESI, APCI or APPI 14 T o convert from ESI, APCI or APPI to the multimode source 1 ...

  • Agilent Technologies G1978B - page 6

    6 Multimode Source for 6100 Series Singl e Quad LC/MS Set-U p Guide Contents ...

  • Agilent Technologies G1978B - page 7

    7 Agilent G1978B Multimode Source fo r 6100 Series Single Quad LC/MS Set-Up Guide Agilent T echnologies 1 Installation Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cabl es 9 Changing Sources 13 T o remove the multimode source 13 T o convert from multimode to ESI, APCI or APPI 14 T o convert from ESI, APCI or AP ...

  • Agilent Technologies G1978B - page 8

    8 Multimode Source for 6100 Series Singl e Quad LC/MS Set-U p Guide 1 Installation Installation Installation This section explains ho w t o insta ll t he G1978B multimode source on a 6100 Series Single Quad LC/MS instrument. The G1978A multimode source is not suppor ted on these instr uments. The 6100 Series Single Quad LC/MS wit h multimode source ...

  • Agilent Technologies G1978B - page 9

    Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 9 Installation 1 Step 2. Install the HV control PCA and cables • V alv e BD-APCI supply , p/nG1960-60802 • V alv e BD-APCI Needle Interlock, p/n G1960-60856 Figure 2 From left to right: G1946-80058, G1960-60802 and G1960-60856 Step 2. Install the HV control PCA and cables 1 Tur n o ...

  • Agilent Technologies G1978B - page 10

    10 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation Step 2. Install the HV control PCA and cables Figure 4 Connecting th e valve PCA to the multimode HV p ower supply . 6 Install t he APCI HV po wer suppl y. The APCI HV po wer supply is located at the e nd of the A UX Mo du le. 7 Connect ribbon cable betw een va lv e P ...

  • Agilent Technologies G1978B - page 11

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 11 Installation 1 Step 2. Install the HV control PCA and cables Figure 6 Connecting the V cap and V chamber cables to the powe r supply . 9 Connect the long ribbon cable, p/n G1960-60802, from the APCI HV po w er sup ply to the va lv e PC A. Figure 7 Connecting the A PCI HV power sup ...

  • Agilent Technologies G1978B - page 12

    12 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation Step 2. Install the HV control PCA and cables Figure 9 Connecting the HV PCA to the chassis. 12 Close t he A UX Module cover and reconnect all cables. 13 Inst all the multimo de source to t he system and c onnect all connectors. Figure 10 Installing th e multimode sou ...

  • Agilent Technologies G1978B - page 13

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 13 Installation 1 Changing Sources Changing Sources T o remove the multimode source Do the follo wing ste ps to remov e the m ultimode source. 1 Set the source temperatures f or vaporizer heater and dr ying gas heater t o minimum values to cool the source. Use the T une > Instr um ...

  • Agilent Technologies G1978B - page 14

    14 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from multimode to ESI, APCI or APPI 10 Place the source sh ipping co ver on the source. T o convert from multimode to ESI, APCI or APPI 1 If the s ource to be inst alled is an APPI source, disconnect the multimode high vo ltage PCA RS-232 serial cable fr om ...

  • Agilent Technologies G1978B - page 15

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 15 Installation 1 To convert from ES I, APCI or APPI to the multimode source T o convert from ESI, APCI or APPI to the multimode source 1 Swi tch to the MSD T une view . 2 Select Instr ument/Set Spray Chamber and set all gas f l o ws and tem pera tur es to 0 . • Dr ying Gas (L/min) ...

  • Agilent Technologies G1978B - page 16

    16 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from ESI, APCI or APPI to the multimode source Figure 11 Standard spray shiel d and capillary cap for ESI or A PCI 10 Remov e the capillar y cap. If need ed, moisten a clean clot h with isopropyl alcohol and wipe the capillar y cap. See Figur e 1 2 . Figure ...

  • Agilent Technologies G1978B - page 17

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 17 Installation 1 To convert from ES I, APCI or APPI to the multimode source Figure 13 Multimode spray shield 13 Screw the multimo de spray shield into the holder for the spra y shield. See Fig ure 1 4 . Figure 14 Multimode spray shie ld installed 14 Remov e the shippi ng co ver from ...

  • Agilent Technologies G1978B - page 18

    18 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from ESI, APCI or APPI to the multimode source Figure 15 Multimode Spray Cha mber 15 Inst all the spray chamber on the spray chamber mount. Figure 16 Multimode source with I-Button 16 Install t he nebulizer on t he mu ltimode source spra y chamber . I-Butto ...

  • Agilent Technologies G1978B - page 19

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 19 Installation 1 To convert from ES I, APCI or APPI to the multimode source Figure 17 No nebulizer on top of the multimode source 17 Connect the 1/8-inch nebulizer gas tu bing from the LC/MS mainframe to the nebulizer gas f itting. See Fi gu re 18 . Figure 18 Nebulizer with ga s tub ...

  • Agilent Technologies G1978B - page 20

    20 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from ESI, APCI or APPI to the multimode source 18 Connect the LC/MS sample tubing to the LC/MS divert er valv e inlet filter . See Figu re 19 on page 20. Figure 19 LC/MS sample tub ing connected to LC/MS in let filter 19 If you are installin g the multimod ...

  • Agilent Technologies G1978B - page 21

    21 Agilent G1978B Multimode Source fo r 6100 Series Single Quad LC/MS Set-Up Guide Agilent T echnologies 2 V erification T o verify sensitivity for 6120 Qu ad - G1978B Multimode, Positiv e SIM Modes 22 T o verify sensitivity for 6130 and 614 0 Quads - G1978B Multimode, Positive SIM Modes 24 T o verify sensitivity for 6130 and 614 0 Quads - G1978B M ...

  • Agilent Technologies G1978B - page 22

    22 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes T o verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes Preparation 1 Transfer 1 mL of 5 ng/µL reserpine (A gilent G2423A) to a 50 mL v olumetric f lask. Use a clean g radu ...

  • Agilent Technologies G1978B - page 23

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 23 Verification 2 To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes Sensitivity V erification for Mult imode in ESI Positive SIM Mode 1 Star t up t he ChemSt ation software. 2 Change the view to MSD T une , and star t an Autotune. After the auto tu ne com ple ...

  • Agilent Technologies G1978B - page 24

    24 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 613 0 and 6140 Quads - G1978B Mu ltimode, Positive SIM Modes When the meth od is finished, a repor t prints showing the signal-t o-noise ratio f or the five blank and five sample peaks, and a blank-subtracted average of the sample peaks. The ...

  • Agilent Technologies G1978B - page 25

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 25 Verification 2 To verify sensitivity for 6130 and 6140 Quad s - G1978B Multimode, Positive SIM Modes Sensitivity V erification for Multim ode in ESI, Positive SIM Mode 1 Star t up t he ChemSt ation software. 2 Change the view to MSD T une , and star t an Autotune. After the auto t ...

  • Agilent Technologies G1978B - page 26

    26 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 613 0 and 6140 Quads - G1978B Mu ltimode, Positive SIM Modes experiments t o achieve maxi mum instrument sensitivity 7 Review the results. When the meth od is finished, a repor t prints showing the signal-t o-noise ratio f or the five blank a ...

  • Agilent Technologies G1978B - page 27

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 27 Verification 2 To verify sensitivity for 6130 and 6140 Quad s - G1978B Multimode, Positive Scan Modes T o verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes Preparation 1 Transfer 1 mL of 5 ng/µL reserpine (A gilent G2423A) to a 50 mL v olumetric f ...

  • Agilent Technologies G1978B - page 28

    28 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 613 0 and 6140 Quads - G1978B Mu ltimode, Positive Scan Modes Sensitivity V erification for Multim ode in ESI, Posi tive Scan Mode 1 Star t the ChemSt ation software. 2 Change the view to MSD T une , and star t an Autotune. After the auto tu ...

  • Agilent Technologies G1978B - page 29

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 29 Verification 2 To verify sensitivity for 6130 and 6140 Quad s - G1978B Multimode, Positive Scan Modes When the me thod is f inished, a repor t prints sho wing t he signal-to-noise ratio f or the f ive blank and five sam ple peaks, and a blank-subtracted av erage of t he sample pea ...

  • Agilent Technologies G1978B - page 30

    30 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for the G1 978B Multimode using Multiple FIA method in Mixed Mode operation T o verify sensitivity for the G1978B Multimode using Multiple FIA method in Mixed Mode operation Preparation 1 Do an autotune if necessar y. After the auto tu ne has co ...

  • Agilent Technologies G1978B - page 31

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 31 Verification 2 To verify sensitivity for the G1 978B Multimode using Multiple FIA method in Mixed Mode operation d Click OK . 8 Repeat f or the MMCheckSL _CI.M data f ile. Use the same subdirector y and change Dat a File to Multi_CI . 9 Repeat f or the MMCheckSL _MX.M data f ile. ...

  • Agilent Technologies G1978B - page 32

    32 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To do an autotune T o do an autotune Tuning t he multimode source is done in MM-APCI mode only. A utotune is done from the same menu as with all sources. • Fr om the MSD Tune view , select Instrument > Autotune menu it em. The tune report will have a header with ...

  • Agilent Technologies G1978B - page 33

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 33 Verification 2 Example of multimode verification report Example of multimode verification report ...

  • Agilent Technologies G1978B - page 34

    34 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification Example of multimode verification report ...

  • Agilent Technologies G1978B - page 35

    35 Agilent G1978B Multimode Source fo r 6100 Series Single Quad LC/MS Set-Up Guide Agilent T echnologies 3 Methods T o setup a method to use the multimode source 36 T o create a method for positive/negative mixed mode operation 37 T o create a method for altern ating ESI and APCI operation 39 Th i s c hap te r d e sc ri bes the ta sks that yo u n e ...

  • Agilent Technologies G1978B - page 36

    36 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3M e t h o d s To setup a method to use the multimode source T o setup a method to use the multimode source T o ha ve y our method use a multimode source, fo llo w these steps: 1 Open the MSD Spray Chamber dialog bo x by clicking Instrument > MSD Spray Chamber in the Met hod and ...

  • Agilent Technologies G1978B - page 37

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 37 Methods 3 To create a method for posit ive/negative mixed mode opera tion T o create a method for positive/negative mixed mode operation 1 Open the MSD Spray Chamber dialog bo x by clicking the Instr ument > Set Up M SD S ig na ls in the Met hod and Run Control vie w . 2 Select ...

  • Agilent Technologies G1978B - page 38

    38 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3M e t h o d s To create a method for posit ive/negative mixed mode op eration Figure 21 Positive/Negativ e polarity switching using Set Up MSD Signals dialog box Polarity options for S ignal 1 and Signal 2 ...

  • Agilent Technologies G1978B - page 39

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 39 Methods 3 To create a method for alternating ESI and APCI operation T o create a method for alternating ESI and APCI operation 1 Open The MSD Spray Chamber dialog bo x by clicking Instrument > MSD Spray Chamber in the Method and Run Control view . 2 Set Met hod Spray Chamber to ...

  • Agilent Technologies G1978B - page 40

    40 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3M e t h o d s To create a method for altern ating ESI and APCI operation 9 Click the OK button. Figure 23 MM-ES and MM- APCI switching using Set Up MSD Si gnals dialog box NOTE In general, use the mi xed mode operation (MM-ES+ APCI setting with Si gnal 1) instead of alternating MM- ...

  • Agilent Technologies G1978B - page 41

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 41 Index Index A autotune , 32 C converting from ESI, APCI or AP PI , 15 converting to E SI or APCI , 14 D diverter valv e inlet filter , 20 E ESI convert from , 15 convert to , 14 I installation , 7 , 21 L LC/MSD sample tubing , 20 M method alternating ESI and APCI , 39 basic setup ...

  • Agilent Technologies G1978B - page 42

    42 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide Index ...

  • Agilent Technologies G1978B - page 43

    ...

  • Agilent Technologies G1978B - page 44

    © Agilent T echnologi es, Inc. 2008 Printed in USA First Editio n, December 20 08 *G1978-90070* G1978-90070 www .agilent.com Agilent T echnologies In This Book This book contains installation, operation, maintenance and troubleshooting instruction fo r the Multimode Source fo r 6100 Series Single Quad LC/MS. ...

Manufacturer Agilent Technologies Category Computer Accessories

Documents that we receive from a manufacturer of a Agilent Technologies G1978B can be divided into several groups. They are, among others:
- Agilent Technologies technical drawings
- G1978B manuals
- Agilent Technologies product data sheets
- information booklets
- or energy labels Agilent Technologies G1978B
All of them are important, but the most important information from the point of view of use of the device are in the user manual Agilent Technologies G1978B.

A group of documents referred to as user manuals is also divided into more specific types, such as: Installation manuals Agilent Technologies G1978B, service manual, brief instructions and user manuals Agilent Technologies G1978B. Depending on your needs, you should look for the document you need. In our website you can view the most popular manual of the product Agilent Technologies G1978B.

Similar manuals

A complete manual for the device Agilent Technologies G1978B, how should it look like?
A manual, also referred to as a user manual, or simply "instructions" is a technical document designed to assist in the use Agilent Technologies G1978B by users. Manuals are usually written by a technical writer, but in a language understandable to all users of Agilent Technologies G1978B.

A complete Agilent Technologies manual, should contain several basic components. Some of them are less important, such as: cover / title page or copyright page. However, the remaining part should provide us with information that is important from the point of view of the user.

1. Preface and tips on how to use the manual Agilent Technologies G1978B - At the beginning of each manual we should find clues about how to use the guidelines. It should include information about the location of the Contents of the Agilent Technologies G1978B, FAQ or common problems, i.e. places that are most often searched by users in each manual
2. Contents - index of all tips concerning the Agilent Technologies G1978B, that we can find in the current document
3. Tips how to use the basic functions of the device Agilent Technologies G1978B - which should help us in our first steps of using Agilent Technologies G1978B
4. Troubleshooting - systematic sequence of activities that will help us diagnose and subsequently solve the most important problems with Agilent Technologies G1978B
5. FAQ - Frequently Asked Questions
6. Contact detailsInformation about where to look for contact to the manufacturer/service of Agilent Technologies G1978B in a specific country, if it was not possible to solve the problem on our own.

Do you have a question concerning Agilent Technologies G1978B?

Use the form below

If you did not solve your problem by using a manual Agilent Technologies G1978B, ask a question using the form below. If a user had a similar problem with Agilent Technologies G1978B it is likely that he will want to share the way to solve it.

Copy the text from the picture

Captcha
New picture

Comments (0)